Jane Hu Coffman
Staff Research Associate
I began working with retinal pigment epithelial (RPE) cells three decades ago. My previous work, under the direction of Dr. Dean Bok, included developing original methods to culture fetal human RPE cells, contributing to the identification of Na/K ATPase pump, chloride channels, the vitamin A receptor, and exploring the complement regulation pathways in cultured human RPE cells. In Radu’s lab, I have been working on characterization of disease models (both mouse and cell-based) to study the ABCA4-mediated RPE cellular dysfunction with an emphasize on the complement system and lipid metabolism. Currently, I oversee all the work involving human fetal- and iPSC-derived RPE culture cells. Using my extensive immunohistochemistry expertise, I am involved in the evaluation of specific markers in tissue from mouse retina, RPE cultured cells, and cadaveric donor eyes. As a senior researcher in the lab, I actively teach our current trainees to apply basic tissue culture protocols and perform immunohistochemistry assays for confocal microscopy analysis.