The Agilent 2100 Bioanalyzer as the first commercially available instrument to use microfluids technology for the analysis of biological samples. It has many advantages over conventional techniques. These advantages include improved data precision and reproducibility, short analysis times, minimal sample consumption, improved automation and integration of complex workflows.
The system provides automated sizing and quantitation information in a digital format. On-chip gel electrophoresis is performed for the analysis of DNA, RNA and proteins.
The chip accommodates sample wells, gel wells and a well for an external standard (ladder). Then, twelve samples (NanoChip kit), or eleven samples (PicoChip kit) and ladder with marker are loaded in each well. Once the wells and channels are filled, the chip becomes an integrated electrical circuit. The 16-pin electrodes of the cartridge are arranged so that they fit into the wells of the chip. Each electrode is connected to an independent power supply that provides maximum control and flexibility.
The software automatically compares the unknown samples to the ladder fragments to determine the concentration of the unknown samples and to identify the ribosomal RNA peaks.
To learn more about this technology, visit the Agilent website
The instrument is operated by core personnel only. We will run the chip and send you a .pdf file containing the results. For each samples, we require a minimum of 5µl per sample.
Please fill up the sample submission form and bring it with you when you drop off your samples.
No need for an appointment, just email us and we will get back to you within 24 hrs.
To submit samples for BioAnalyzer, please complete the form:
We also offer DNA, RNA sample concentration measurements using the Thermo Scientific NanoDrop 2000 spectrophotometer.
For information regarding our services, prices and samples submissions, please contact Dr. Emmanuelle Faure-Kumar at csbm@mednet.ucla.edu
