Protocols

• DNA Extraction from Mouse Tail Biopsies - Phenol/Chloroform Method View PDF
• DNA Extraction from Mouse Tail Biopsies - Ammonium Acetate Method View PDF

In vivo

• Cleared Skeletal Preparations View Link
• beta-galactosidase staining of whole embryos View PDF
• Paraffin-embedding of cartilaginous tissues View PDF
• Alcian blue/nuclear fast red staining of Paraffin Sections View PDF
• Hematoxylin and Eosin staining of Paraffin Sections View PDF
• Safranin-O staining of cartilage View PDF
• TRAP staining of Paraffin Sections View PDF
• General protocol for immunostaining of Paraffin Sections View PDF
• Immunostaining of Paraffin Sections with tyramide amplification View PDF

In vitro

• Isolation of mouse primary sternal chondrocytes View PDF
• Isolation of mouse primary calvarial osteoblasts View PDF
• Isolation of mouse primary preadipocytes View PDF
• Isolation of mouse embryonic fibroblasts View PDF
• Limb bud micromass cultures View PDF
• Alcian blue staining of limb bud micromass cultures View PDF
• Oil red O staining of adipocytes View PDF
• Isolation of protein lystates from isolated primary chondrocytes View PDF
• SDS-PAGE Western Blot View PDF