• UCLA Health
  • myUCLAhealth
  • School of Medicine
UCLA Orthopaedic Surgery

UCLA Orthopaedic Surgery

UCLA Orthopaedic Surgery
  • About Us
    • Equity, Diversity & Inclusion
    • Ortho in the News
    • Research Publications
    • Make a Gift
    • Contact Us
  • Patient Resources
    • Request an Appointment
    • Billing and Insurance
    • Health Library
    • Insurances Accepted
    • New Patients
    • Preparing for Your Appointment
    • Preparing for Your Inpatient Surgery at UCLA Santa Monica Hospital
    • Interactive Procedure Videos
    • Maps / Directions
    • Webinars
    • Contact Us
  • Clinical Programs
    • After-Hours Clinic
    • Cerebral Palsy
    • Foot & Ankle Surgery
    • General Orthopaedics
    • Hand Surgery
    • Joint Replacement
    • Oncology
    • Pediatric Orthopaedics
    • Shoulder and Elbow Surgery
    • Spinal Disorders
    • Sports Medicine
    • Trauma
  • Our Locations
  • Research
    • Message from Vice Chair
    • Research Faculty
    • Billi Research Lab
    • UCLA Biomechanics Lab
    • International Skeletal Dysplasia Registry (ISDR)
    • Lyons Research Lab
    • Vitamin D Research Lab
    • Clinical Trials
    • Grants & Awards
    • Musculoskeletal Calendar
  • Education
    • Our Affiliates
    • Visiting MS4 Students
    • Residency Program
    • Fellowships
    • Post-Doctoral Research Training Programs
    • Grand Rounds
    • Alumni
  • Our Expert Team
    • Physicians
    • Physician Assistants & Nurse Practitioners
    • Research Faculty
  • UCLA Health
  • myUCLAhealth
  • School of Medicine

UCLA Orthopaedic Surgery

Lyons Research Lab

Lyons Research Lab

Lyons Research Lab

  • Research
    • BMP Research
    • CCN Research
    • Matricellular Proteins
    • Osteoarthritis
    • The Growth Plate
  • Lab Members
  • Protocols
    • Protocols Cleared Skeletal Preps
  • Publications
  • Useful Links
  • Contact Us
  • Research
  • Lab Members
  • Protocols
  • Publications
  • Useful Links
  • Contact Us
  • Protocols Cleared Skeletal Preps
  1. Home
  2. Research
  3. Lyons Research Lab
  4. Protocols

Protocols

Share this
  • DNA Extraction from Mouse Tail Biopsies - Phenol/Chloroform Method View PDF
  • DNA Extraction from Mouse Tail Biopsies - Ammonium Acetate Method View PDF

In vivo

  • Cleared Skeletal Preparations View Link
  • beta-galactosidase staining of whole embryos View PDF
  • Paraffin-embedding of cartilaginous tissues View PDF
  • Alcian blue/nuclear fast red staining of Paraffin Sections View PDF
  • Hematoxylin and Eosin staining of Paraffin Sections View PDF
  • Safranin-O staining of cartilage View PDF
  • TRAP staining of Paraffin Sections View PDF
  • General protocol for immunostaining of Paraffin Sections View PDF
  • Immunostaining of Paraffin Sections with tyramide amplification View PDF

In vitro

  • Isolation of mouse primary sternal chondrocytes View PDF
  • Isolation of mouse primary calvarial osteoblasts View PDF
  • Isolation of mouse primary preadipocytes View PDF
  • Isolation of mouse embryonic fibroblasts View PDF
  • Limb bud micromass cultures View PDF
  • Alcian blue staining of limb bud micromass cultures View PDF
  • Oil red O staining of adipocytes View PDF
  • Isolation of protein lystates from isolated primary chondrocytes View PDF
  • SDS-PAGE Western Blot View PDF
Like Us on Facebook Follow Us on Twitter Subscribe to Our Videos on YouTube Follow us on Instagram Connect with Us on LinkedIn Follow us on Pinterest
UCLA Health hospitals ranked best hospitals by U.S. News & World Report
  • UCLA Health
  • Find a Doctor
  • School of Medicine
  • School of Nursing
  • UCLA Campus
  • Directory
  • Newsroom
  • Subscribe
  • Patient Stories
  • Giving
  • Careers
  • Volunteer
  • International Services
  • Privacy Practices
  • Nondiscrimination
  • Billing
  • Health Plans
  • Emergency
  • Report Broken Links
  • Terms of Use
  • 1-310-825-2631
  • Locations / Directions
  • Contact Us
  • Your Feedback
  • Report Misconduct
  • Get Social
  • Sitemap
Like Us on Facebook Follow Us on Twitter Subscribe to Our Videos on YouTube Follow us on Instagram Connect with Us on LinkedIn Follow us on Pinterest

Sign in to myUCLAhealth

Learn more about myUCLAhealth