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DNA Extraction from Mouse Tail Biopsies - Phenol/Chloroform Method
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DNA Extraction from Mouse Tail Biopsies - Ammonium Acetate Method
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In vivo
Cleared Skeletal Preparations
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beta-galactosidase staining of whole embryos
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Paraffin-embedding of cartilaginous tissues
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Alcian blue/nuclear fast red staining of Paraffin Sections
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Hematoxylin and Eosin staining of Paraffin Sections
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Safranin-O staining of cartilage
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TRAP staining of Paraffin Sections View PDF
General protocol for immunostaining of Paraffin Sections
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Immunostaining of Paraffin Sections with tyramide amplification
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In vitro
Isolation of mouse primary sternal chondrocytes
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Isolation of mouse primary calvarial osteoblasts
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Isolation of mouse primary preadipocytes
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Isolation of mouse embryonic fibroblasts
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Limb bud micromass cultures
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Alcian blue staining of limb bud micromass cultures
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Oil red O staining of adipocytes
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Isolation of protein lystates from isolated primary chondrocytes
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SDS-PAGE Western Blot
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