Dr. Dino Di Carlo's research focuses on engineering of microfluidic and nanoparticle systems for the analysis of single cells for both improving cell-based diagnostics and automating cell biology. His group takes advantage of inertial fluid dynamic effects in particular to engineer simple systems for translational applications. Projects include:
- measuring the mechanical properties of single cells at thousands of cells per second for label-free cancer screening
- an automated cellular sample preparation instrument called the centrifuge-on-a-chip to prepare fluid samples from patients for improved cytological and molecular diagnostics
- nanoparticle-based manipulation of intracellular environments using patterned magnetic substrates to study how local controlled signals contribute to cell response in normal and diseased cells.
Che J, Yu V, Garon EB, Goldman JW, Di Carlo D. Biophysical isolation and identification of circulating tumor cells. Lab Chip. 2017 Apr 11;17(8):1452-1461. doi: 10.1039/c7lc00038c.
Murray C, Pao E, Tseng P, Aftab S, Kulkarni R, Rettig M, Di Carlo D. Quantitative Magnetic Separation of Particles and Cells Using Gradient Magnetic Ratcheting. Small. 2016 Apr 13;12(14):1891-9. doi: 10.1002/smll.201502120. Epub 2016 Feb 17.
Che J, Yu V, Dhar M, Renier C, Matsumoto M, Heirich K, Garon EB, Goldman J, Rao J, Sledge GW, Pegram MD, Sheth S, Jeffrey SS, Kulkarni RP, Sollier E, Di Carlo D. Classification of large circulating tumor cells isolated with ultra-high throughput microfluidic Vortex technology. Oncotarget. 2016 Mar 15;7(11):12748-60. doi: 10.18632/oncotarget.7220.
Dhar M, Pao E, Renier C, Go DE, Che J, Montoya R, Conrad R, Matsumoto M, Heirich K, Triboulet M, Rao J, Jeffrey SS, Garon EB, Goldman J, Rao NP, Kulkarni R, Sollier-Christen E, Di Carlo D. Label-free enumeration, collection and downstream cytological and cytogenetic analysis of circulating tumor cells. Sci Rep. 2016 Oct 14;6:35474. doi: 10.1038/srep35474.
Gossett DR, Tse HT, Lee SA, Ying Y, Lindgren AG, Yang OO, Rao J, Clark AT, Di Carlo D. Hydrodynamic stretching of single cells for large population mechanical phenotyping. Proc Natl Acad Sci U S A. 2012 May 15;109(20):7630-5. Epub 2012 Apr 30.