Glioblastoma is still the most common and most aggressive form of adult brain cancer, and unfortunately treatments remain limited. Using single-cell based approaches, Dr. Bhaduri is seeking to understand how different cell populations within a tumor contribute to tumor origin, progression, and recurrence. In addition to using primary patient tumor resections, Dr. Bhaduri is also working with and developing new ways of culturing these tumors in vitro while maintaining their original heterogeneity. This work is complementary to the work in the lab studying normal neural progenitor cell fate specification, as developmental trajectories are frequently co-opted during tumorigenesis.
Parker KR, Migliorini D, Perkey E, Yost KE, Bhaduri A, Bagga P, Haris M, Wilson NE, Liu F, Gabunia K, Scholler J, Montine TJ, Bhoj VG, Reddy R, Mohan S, Maillard I, Kriegstein AR, June CH, Chang HY, Posey Jr AD, Satpathy AT. “Single-Cell Analyses Identify Brain Mural Cells Expressing CD19 as Potential Off-Tumor Targets for CAR-T Immunotherapies.” Cell, 2020. Oct 1;183(1):126-142.e17. doi: 10.1016/j.cell.2020.08.022.
Ji AL, Rubin AJ, Thrane K, Jiang S, Reynolds DL, Meyers RM, Guo MG, George BM, Bhaduri A, Mollbrink A, Bergenstråhle J, Larsson L, Meyers J, Hollmig ST, Aasi SZ, Nolan GP, Lundeberg J, Khavari PA. “Multimodal Analysis of Composition and Spatial Architecture in Human Squamous Cell Carcinoma.” Cell, 2020. Jul 23;182(2):497-514.e22. doi: 10.1016/j.cell.2020.05.039.
Bhaduri A*, Di Lullo E*, Jung D, Müller S, Crouch EE, Sandoval Espinosa C, Ozawa T, Alvarado B, Spatazza J, Cadwell CR, Wilkins G, Velmeshev D, Liu SJ, Malatesta M, Andrews MG, Mostajo-Radji MA, Huang EJ, Lim DA, Nowakowski TJ, Diaz A, Raleigh DR, Kriegstein AR. “Outer Radial Glia-like Cancer Stem Cells Contribute to Heterogeneity of Glioblastoma.” Cell Stem Cell, 2020 Jan 02; 26 (1), 48-63.e6. doi: 10.1016/j.stem.2019.11.015 *co-first author
Kovalski J, Bhaduri A, Zehnder AM, Neela PH, Che YC, Wozniak GG, Khavari PA. “The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.” Molecular Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 10.1016/j.molcel.2018.12.001.
Nagy A, Bhaduri A, Shahmarvand N, Shahryari J, Zehnder JL, Warnke RA, Mughal T, Ali S, Ohgami RS. “Next-generation sequencing of idiopathic multicentric and unicentric Castleman disease and follicular dendritic cell sarcomas.” Blood Advances. 2018. Mar 13(5): 481-491. doi: 10.1182/bloodadvances.2017009654.