Long Read Sequencing (Third Generation) and Next Generation Sequencing (Second Generation)
DNA and RNA sequencing is the process of determining the nucleotide order of a given DNA fragment. The nucleotide sequence encodes the necessary information that allows living things to survive and reproduce. Determining the sequence is therefore useful in researching into how organisms live. Our core facility provides many sequencing options to meet your individual needs, utilizing (a) Oxford NanoPore GridIONx5 Long-Read Sequencing (by reading the nucleotide sequences at the single molecule level), and (b) Illumina Short-Read Sequencing (by massive parallel sequencing of millions of DNA fragments and yielding substantially more throughput reads quickly).
The Chromium 10x genomics 3’ Single Cell Gene Expression Solution provides a comprehensive, scalable solution for cell characterization and gene expression profiling of hundreds to tens of thousands of cells. The Chromium Single Cell Immune Profiling simultaneously examines the cellular context of the adaptive immune response and immune repertoires of hundreds to tens of thousands of T and B cells in human or mouse tissue on a cell-by-cell basis. The newest one is the Chromium Single Cell ATAC (Assay for Transposase Accessible Chromatin), which accelerates the understanding of the regulatory landscape of the genome, providing insights into cell variability.
The human genome contains approximately 25,000 genes, and each cell of the human body contains a copy of every gene. At a given moment, each cell has a combination of genes that are turned on and off. This combination of genes that are "on/off" can change at any time depending on internal and external stimuli.
Thus, it can be very important to determine which genes are turned on and off. Microarray analysis allows us to do this. The TCGB is equipped with various platforms to meet your research needs.
The TCGB has various academic and commercial data mining software and other resources for genomic data analysis, including Partek flow, VarSeq, Ingenuity Pathway Analysis, Falcon automated genomic analysis system,Hoffman2 data analysis clusters, UCLA CASS server, Illumina basespace Sequencing Hub professional, Amazon cloud HIPAA compliant research computing, and storage system server.
Educational workshops are offered annually. Our core facility also provides RNA/DNA extraction; RNA/DNA quality control and quantification; cDNA synthesis for quantitative-PCR; and QRT-PCR.
