UIC uses state-of-the art, robust, accurate and reliable molecular methods for genotyping HLA Class I and Class II and MICA antigens.
Individual methods for HLA typing result in varying levels of resolution- low, intermediate or high (Table 1).
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Table 1. HLA Typing Methods, Resolution, Results and Potential Applications |
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Method |
Resolution |
Result |
Potential Applications |
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Intermediate |
A*02:AGFGE1 |
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High |
A*02:01:01G |
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High: Class I- Full Gene sequence Class II- Near Full Gene sequence |
A*02:02:01 |
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1. Intermediate resolution HLA typing results by SSO include a NMDP string that defines the list |
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Reverse sequence specific oligonucleotide (SSO) hybridization is used to determine HLA-A, -B, -C, -DRB, -DQA, -DQB, -DPA and -DPB locus types at intermediate and higher than intermediate levels of resolution. This technology is used for high volume testing allowing for relatively low-cost typing for bone marrow donor drives or other applications involving large sample numbers. Special volume pricing and terms may apply.
Sanger Sequence-based Typing (SBT) provides high resolution HLA typing for HLA-A, -B, -C, -DR, -DQ and -DP locus alleles. SBT is used when high resolution typing is important as in donors and recipients of stem cell transplants.
High Resolution HLA Typing by Next Generation Sequencing (NGS) provides unambiguous, phase-defined, first pass HLA typing results. Our strategy for HLA typing with NGS allows for full gene sequencing for HLA-A, B, C, DQA1 and DPA1 loci, and long range sequencing from exon 2 through partial-exon 4 (including introns) for HLA-DR, DQ and DP. In addition, full gene sequencing for HLA-DR, DQ and DP antigens is currently under validation. This method of typing enables reporting of allele level results to a minimum of three nomenclature fields for HLA Class I, DQA1 and DPA1 genes and two nomenclature fields for Class II genes, or minimum G group allele level typing. The long range NGS typing provides the highest HLA typing resolution that is important for donor selection for stem cell transplants. In addition, it can be used to define HLA alleles of solid organ transplant donors to aid in the understanding of allele specific antibodies displayed by the recipient.
HLA typing for disease associations and drug hypersensitivity is generally performed via SSO(link to information).
MICA genotyping targets MICA sequence polymorphisms by SSO(link to information). MICA typing can be helpful in determining whether patients are matched or mismatched for MICA with their donors, and to facilitate identification of donor specific MICA antibodies.
Killer Cell Ig-Like Receptors (KIRs) are used by NK cells to distinguish abnormal cells from healthy cells. KIR genotyping performed by SSO(link to information) identifies the presence and absence of 15 distinct KIR genes. Based on the result, the KIR A or B haplotypes can be assigned. Donor KIR B content is associated with positive outcomes after stem cell transplant. Presence of maternal KIR AA haplotypes are also associated with preeclampsia, fetal growth restriction and recurrent miscarriage in pregnant women.
