The Immune Assessment Core (IAC) is a CLIA certified laboratory (#045D0642433) providing comprehensive immunological testing services for basic, clinical, and translational studies. The IAC provides both standardized and customized multi-parameter flow cytometry, multiplexed immunoassays, ultra-sensitive immunoassay technology (Simoa Quanterix), and cellular immune function assays to evaluate the innate and adaptive immune status of study subjects. The Core uses high-throughput technologies to maximize data output using small sample volumes. We work closely with our clients to assist with data analysis and interpretation to maximize knowledge gained.
Validated tests include comprehensive immunophenotyping panels, multiplexed cytokine/chemokine detection in body fluids or culture supernatants, single molecule assays (Simoa), several ELISA assays, allo- and virus-specific T cell activation, T and B cell proliferation to antigens and mitogens, NK cell cytotoxicity, and granulocyte/monocyte oxidative burst. These assays can be used to aid the diagnosis of primary immunodeficiency and for immune monitoring purposes. In addition to our various validated assays, the core performs customized assay development to meet the investigators’ needs.
The IAC is actively involved in projects aimed at standardizing immune assessment assays, including the NIH CTOT harmonization consortium and the FOCIS/NIH sponsored HIPC validation project. These consortia are focused on developing and implementing standardized protocols for testing and data analysis.
Kyle Valiente Almendras was recruited as the new SRA in the Immune Assessment Core
Cappelletti M, Presicce P, Feiyang M, Senthamaraikannan P, Miller LA, Pellegrini M, Sim MS, Jobe AH, Divanovic S, Way SS, Chougnet CA, Kallapur SG. The induction of preterm labor in rhesus macaques is determined by the strength of immune response to intrauterine infection. PLoS Biol. 2021 Sep 8;19(9):e3001385. doi: 10.1371/journal.pbio.3001385. PMID: 34495952; PMCID: PMC8452070.
Chang YL, Rossetti M, Gjertson DW, Rubbi L, Thompson M, Montoya DJ, Morselli M, Ruffin F, Hoffmann A, Pellegrini M, Fowler VG Jr, Yeaman MR, Reed EF; with the MRSA Systems Immunology Group. Human DNA methylation signatures differentiate persistent from resolving MRSA bacteremia. Proc Natl Acad Sci U S A. 2021 Mar 9;118(10):e2000663118. doi: 10.1073/pnas.2000663118. PMID: 33649198; PMCID: PMC7958259.
Sosa RA, Terry AQ, Kaldas FM, Jin YP, Rossetti M, Ito T, Li F, Ahn RS, Naini BV, Groysberg VM, Zheng Y, Aziz A, Nevarez-Mejia J, Zarrinpar A, Busuttil RW, Gjertson DW, Kupiec-Weglinski JW, Reed EF. Disulfide High-Mobility Group Box 1 Drives Ischemia-Reperfusion Injury in Human Liver Transplantation. Hepatology. 2021 Mar;73(3):1158-1175. doi: 10.1002/hep.31324. Epub 2020 Oct 30. PMID: 32426849.